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activina  (R&D Systems)


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    Structured Review

    R&D Systems activina
    Activina, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+recombinant+activina/Recombinant+Human+follistatin-resistant+Activin+A%2CAF+Protein/pm41413587-70-35-36
    Average 93 stars, based on 4 article reviews
    activina - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Recombinant:

    Article Title: The Bone Morphogenetic Protein Type Ib Receptor Is a Major Mediator of Glial Differentiation and Cell Survival in Adult Hippocampal Progenitor Cell Culture
    Article Snippet: For induction of Smad1/5-phosphorylation, human recombinant BMP2 (R&D Systems, Minneapolis, MN) or human recombinant BMP6 (kindly provided by Creative Biomolecules, Boston, MA) were added to the medium for 3 h before cell lysis and Western blotting on day in vitro (DIV)4. .. For induction of Smad2-phosphorylation, human recombinant activinA (R&D Systems) or human recombinant TGF-β (R&D Systems) were added to the medium for 3 h before cell lysis and Western blotting on DIV4. .. Noggin (R&D Systems) was added to some of the cultures on DIV2 and DIV4.

    Article Title: Eomes and Brachyury control pluripotency exit and germ layer segregation by changes of chromatin state
    Article Snippet: For embryoid body (EB) formation, 200 cells in 40 μl ESGRO Complete Basal medium were grown in ultra-low attachment 96-well plates (Greiner BioOne) for 2 days until single EB had formed in every well. .. EBs were subsequently transferred into 60 mm non-adhesive dishes and allowed to further differentiate in ESGRO Complete Basal medium with 30 ng/ml human recombinant ActivinA (ActA, R&D systems). .. After 3 days of ActA treatment EBs were plated on fibronectin (20 μg/ml) coated 8-well μ-Slides (Ibidi, 80826) and grown in ESGRO Complete Basal medium supplemented with 5% FBS for additional 2 days.

    Article Title: Eomes restricts Brachyury functions at the onset of mouse gastrulation.
    Article Snippet: For embryoid body (EB) formation, 200 cells in 40 ml ESGRO Complete Basal medium were grown in ultra-low attachment 96-well plates (Greiner BioOne) for 2 days until EBs had formed. .. EBs were transferred into 60mm non-adhesive dishes and differentiated in ESGRO Complete Basal medium with 30 ng/ml human recombinant ActivinA (ActA, R&D systems) for 3 days. .. For ChIP-seq samples, 2.5x10^5 cells were seeded in 10 ml ESGRO Complete Basal medium into 100 mm non-adhesive dishes.

    Article Title: Eomes restricts Brachyury functions at the onset of mammalian gastrulation
    Article Snippet: For embryoid body (EB) formation, 200 cells in 40 μl ESGRO Complete Basal medium were grown in ultra-low attachment 96-well plates (Greiner BioOne) for 2 days until EBs had formed. .. EBs were transferred into 60 mm non-adhesive dishes and differentiated in ESGRO Complete Basal medium with 30 ng/ml human recombinant ActivinA (ActA, R&D systems) for 3 days. .. For ChIP-seq samples, 2.5×10^5 cells were seeded in 10 ml ESGRO Complete Basal medium into 100 mm non-adhesive dishes.

    Lysis:

    Article Title: The Bone Morphogenetic Protein Type Ib Receptor Is a Major Mediator of Glial Differentiation and Cell Survival in Adult Hippocampal Progenitor Cell Culture
    Article Snippet: For induction of Smad1/5-phosphorylation, human recombinant BMP2 (R&D Systems, Minneapolis, MN) or human recombinant BMP6 (kindly provided by Creative Biomolecules, Boston, MA) were added to the medium for 3 h before cell lysis and Western blotting on day in vitro (DIV)4. .. For induction of Smad2-phosphorylation, human recombinant activinA (R&D Systems) or human recombinant TGF-β (R&D Systems) were added to the medium for 3 h before cell lysis and Western blotting on DIV4. .. Noggin (R&D Systems) was added to some of the cultures on DIV2 and DIV4.

    Western Blot:

    Article Title: The Bone Morphogenetic Protein Type Ib Receptor Is a Major Mediator of Glial Differentiation and Cell Survival in Adult Hippocampal Progenitor Cell Culture
    Article Snippet: For induction of Smad1/5-phosphorylation, human recombinant BMP2 (R&D Systems, Minneapolis, MN) or human recombinant BMP6 (kindly provided by Creative Biomolecules, Boston, MA) were added to the medium for 3 h before cell lysis and Western blotting on day in vitro (DIV)4. .. For induction of Smad2-phosphorylation, human recombinant activinA (R&D Systems) or human recombinant TGF-β (R&D Systems) were added to the medium for 3 h before cell lysis and Western blotting on DIV4. .. Noggin (R&D Systems) was added to some of the cultures on DIV2 and DIV4.



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